Cardiac calcium channels in planar lipid bilayers. L-type channels and calcium-permeable channels open at negative membrane potentials.

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Cardiac calcium channels in planar lipid bilayers. L-type channels and calcium-permeable channels open at negative membrane potentials

Planar lipid bilayer recordings were used to study Ca channels from bovine cardiac sarcolemmal membranes. Ca channel activity was recorded in the absence of nucleotides or soluble enzymes, over a range of membrane potentials and ionic conditions that cannot be achieved in intact cells. The dihydropyridine-sensitive L-type Ca channel, studied in the presence of Bay K 8644, was identified by a de...

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Introduction: We investigated the role of ATP-sensitive potassium channels and L-type calcium channels in morphine-induced hyperalgesia after nociceptive sensitization. Methods: We used a hotplate apparatus to assess pain behavior in male NMRI mice. Nociceptive sensitization was induced by three days injection of morphine and five days of drug free. On day 9 of the schedule, pain behavior te...

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Sodium Channels in Planar Lipid Bilayers

Single channel currents of sodium channels purified from rat brain and reconstituted into planar lipid bilayers were recorded . The kinetics of channel gating were investigated in the presence of batrachotoxin to eliminate inactivation and an analysis was conducted on membranes with a single active channel at any given time . Channel opening is favored by depolarization and is strongly voltage ...

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Calcium-permeable channels in HeLa cells.

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Calcium-permeable ion channels in pain signaling.

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ژورنال

عنوان ژورنال: Journal of General Physiology

سال: 1988

ISSN: 0022-1295,1540-7748

DOI: 10.1085/jgp.92.1.27